Study reveals how bacteria use ancient self-repair mechanisms

Study reveals how bacteria use ancient self-repair mechanisms

HAS) Sodium floating strain was repeatedly passaged on either Na+LB (~100mM [Na+]) or k+LB (~15mM [Na+]) even. Flares suggesting a possible highly mobile variant were passaged and sent for sequencing. (B) Processed E. coli trunks pots and potsλreceived from E. coli RP437 via no-SCAR or λ-Red recombination were smeared on soft agar (colored background, yellow: Na+; blue: k+) over a period of 18 days, and eight lines (L1 to L8) were selected for further study, each consisting of five members (i.e. L1.3 indicates the first line and the third passage). Buoyancy at high or low sodium levels is indicated by a yellow or blue ring corresponding to swim size on the Swim Plate (C). Lack of exercise on K+ Soft agar is represented by a blue dot indicating colony growth only. Colonies that were not mobile in K.+ Plates were confirmed with further incubation (Fig. S3C). Red and boxed lineage member designations indicate availability of WGS and RNA-seq data (including pots). SNPs identified relative to the Pots reference genome are annotated next to each respective lineage member and in Table S1. Highlighted genes except poma and potB: pita bread (Metal phosphate:H+ to symport), flgl (Flagellar Hook Filament Junction Protein 2), flM (flagellar motor switch protein), cdsA (cardiolipin diglyceride synthase), icd (isocitrate dehydrogenase) and rrsG (16S ribosomal RNA). Scale bar, 10mm. (vs) Summary of the directed evolution experiment. N / A+ (left) and k+ (right) Soft agar plates inoculated with a 1 μL aliquot of the glycerol stock of each strain indicated in (A) (except RP437) and arranged in the same order as (A). (D) phylogeny of wordB over 82 species with ancestral reconstruction at G20 site. G20 is preserved in the vibrio sp. clade. The complete phylogeny is shown in Fig. 1. S14. Recognition: scientific advances (2022). DOI: 10.1126/sciadv.abq2492″ width=”800″ height=”530″/>
Directional evolution of the flagella motor.(HAS) Sodium floating strain was repeatedly passaged on either Na+LB (~100mM [Na+]) or k+LB (~15mM [Na+]) even. Flares suggesting a possible highly mobile variant were passaged and sent for sequencing. (B) Processed E. coli trunks pots and potsλreceived from E. coli RP437 via no-SCAR or λ-Red recombination were smeared on soft agar (colored background, yellow: Na+; blue: k+) over a period of 18 days, and eight lines (L1 to L8) were selected for further study, each consisting of five members (i.e. L1.3 indicates the first line and the third passage). Buoyancy at high or low sodium levels is indicated by a yellow or blue ring corresponding to swim size on the Swim Plate (C). Lack of exercise on K+ Soft agar is represented by a blue dot indicating colony growth only. Colonies that were not mobile in K.+ Plates were confirmed with further incubation (Fig. S3C). Red and boxed lineage member designations indicate availability of WGS and RNA-seq data (including pots). SNPs identified relative to the Pots reference genome are annotated next to each respective lineage member and in Table S1. Highlighted genes except poma and potB: pita bread (Metal phosphate:H+ to symport) flgl (Flagellar Hook Filament Junction Protein 2), flM (flagellar motor switch protein), cdsA (cardiolipin diglyceride synthase), icd (isocitrate dehydrogenase) and rrsG (16S ribosomal RNA). Scale bar, 10mm. (vs) Summary of the directed evolution experiment. N / A+ (left) and k+ (right) Soft agar plates inoculated with a 1 μL aliquot of the glycerol stock of each strain indicated in (A) (except RP437) and arranged in the same order as (A). (D) phylogeny of wordB over 82 species with ancestral reconstruction at G20 site. G20 is preserved in the vibrio sp. clade. The complete phylogeny is shown in Fig. 1. S14. Recognition: scientific advances (2022). DOI: 10.1126/sciadv.abq2492

A new study led by scientists at UNSW Sydney reveals how nature’s oldest wheel, found in bacteria, can repair itself during difficult times.

The results published today in scientific advancesshow how the flagellum — the ancient motor that powers bacteria’s ability to swim — can also help these tiny organisms adapt to conditions in which their motility is compromised.

Bacteria are one of the oldest living organisms on earth. They are tiny single-celled organisms found in every habitat, including the human body — where there are more bacterial cells than human cells.

Being able to swim is crucial to how bacteria survive and spread. However, little is known about how the engines that power their movement help organisms adapt to hostile environments.

The researchers from the School of Biotechnology and Biomolecular Sciences are the first in the world to use CRISPR gene-editing technology to alter a flagellar motor. They used synthetic biology techniques to engineer a sodium engine into the genome to create a sodium-powered swimming bacterium. They then tested and tracked the bacteria’s ability to adapt when the environment was low in sodium.

Sodium is an ion, meaning it carries a charge. It is this charge that drives the flagellar motor through stators or ion channels.

The team found that the stators were able to quickly self-repair the flagellar motor and restore motion. These findings could lead to new advances in the fields of biology and medicine.

“We have shown that environmental changes can cause ion channels to respond rapidly,” said the publication’s lead author, Dr. Pietro Ridone.

“So the CRISPR edits also come back quickly, and the flagellar motor develops and then regulates itself,” said Dr. ridone.

“That we immediately saw mutations directly on the stators is surprising and also inspires many of our future research plans in this area.”

The power of the molecular machinery

The human body contains around 10,000 different types of molecular machines that power a range of biological functions from energy conversion to movement.

The technology of a bacterial motor far surpasses what humans can synthesize at the nanoscale. One-millionth the size of a grain of sand, it can self-assemble and rotate up to five times faster than a Formula 1 engine.

“The engine that powers bacterial swimming is a marvel of nanotechnology,” said Associate Professor Matthew Baker, a co-author of the paper. “It’s the absolute flagship for ancient and very sophisticated molecular machines.”

A/Prof. Baker said the study’s results could help us better understand, in mechanistic detail, the origin of molecular motors – how they came together and how they adapt.

“These ancient parts are a powerful system for studying evolution in general and the origins and development of agility.”

A/Prof. Baker says the results will shed light on how synthetic biology can help create new molecular motors. The results may also have application in understanding antimicrobial resistance and disease virulence.

“By shedding more light on the ancient history of life, we gain knowledge to create tools that can help improve our future,” A/Prof. said baker. “It can also lead us to insights into how bacteria might adapt under future climate change scenarios.”

More information:
Pietro Ridone et al., The rapid evolution of flagellar ion selectivity in experimental populations of E. coli, scientific advances (2022). DOI: 10.1126/sciadv.abq2492

Provided by the University of New South Wales

Quote: Study Uncovers How Bacteria Use Ancient Mechanisms for Self-Repair (2022, November 23), Retrieved November 23, 2022 from https://phys.org/news/2022-11-uncovers-bacteria-ancient-mechanisms-self -repair.html

This document is protected by copyright. Except for fair trade for the purpose of private study or research, no part may be reproduced without written permission. The content is for informational purposes only.


#Study #reveals #bacteria #ancient #selfrepair #mechanisms

Leave a Comment

Your email address will not be published. Required fields are marked *